plastic vial  (Greiner Bio)

Journal: STAR Protocols

Article Title: Protocol for cell-specific RNA expression profiling and lipidomics analyses of Drosophila melanogaster intestinal progenitor cells

doi: 10.1016/j.xpro.2025.104188

Figure Lengend Snippet: A visual guide to efficiently dissect the Drosophila melanogaster midgut (1) Two tubes containing 20 male or female flies each are transferred to an empty tube and anesthetized on ice. (2) Flies are individually dissected in 1×PBS-DEPC: the head is removed, (4) followed by the legs, (5) then the abdomen is separated from the thorax. (6) The gut is extracted via the rectum, (7) cleaned of the sex organs, and (8) trimmed to remove the foregut and hindgut. (9) The isolated midgut is immediately placed in a 1.5 mL tube with 1×PBS-DEPC and returned on ice. (10) This procedure is repeated until 100 male and 100 female midguts are collected in a single tube.

Article Snippet: Drosophila vial plugs , Genesee Scientific , 49-102-VWR.

Techniques: Isolation

Journal: STAR Protocols

Article Title: Protocol for cell-specific RNA expression profiling and lipidomics analyses of Drosophila melanogaster intestinal progenitor cells

doi: 10.1016/j.xpro.2025.104188

Figure Lengend Snippet: A visual summary of the key stages of dissection, FACS, and transcriptomic library or lipidomics preparation Drosophila midguts are collected, digested, and dissociated cells are sorted by FACS (step 1). Sorted cells are either immediately processed for RNA extraction to generate transcriptomic libraries or frozen for lipidomic analysis. The left side of the image (step 2) outlines the lipidomic pipeline: lipids are extracted, the lysate is transferred into an LC-MS vial and run through a tandem liquid chromatography and mass spectrometry unit. Data is collected, lipids are classified using the Agilent MassHunter Workstation, and statistical analysis is performed with MetaboAnalyst. The right side of the image (step 3) outlines the transcriptomic library pipeline: RNA is extracted, reverse transcribed into cDNA, amplified, fragmented, ends blunted, and poly adenylated. Finally, the fragments are amplified again after the addition of an adapter (multiplexing tool used in Next Generation Sequencing), and then quality controlled/quantified.

Article Snippet: Drosophila vial plugs , Genesee Scientific , 49-102-VWR.

Techniques: Dissection, RNA Extraction, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Reverse Transcription, Amplification, Multiplexing, Next-Generation Sequencing